ORIGINAL ARTICLE
Identification of a novel recessive gene for resistance to powdery mildew (Blumeria graminis f. sp. hordei) in barley (Hordeum vulgare)
Urszula Piechota,
Piotr Słowacki,
Paweł C. Czembor,
Corresponding Author
Urszula Piechota
Plant Breeding and Acclimatization Institute – National Research Institute, Błonie, Poland
Correspondence
Urszula Piechota, Plant Breeding and Acclimatization Institute – National Research Institute, Radzików, 05-870 Błonie, Poland.
Email: u.piechota@ihar.edu.pl
Search for more papers by this authorPiotr Słowacki
Plant Breeding and Acclimatization Institute – National Research Institute, Błonie, Poland
Search for more papers by this authorPaweł C. Czembor
Plant Breeding and Acclimatization Institute – National Research Institute, Błonie, Poland
Search for more papers by this authorUrszula Piechota,
Piotr Słowacki,
Paweł C. Czembor,
Corresponding Author
Urszula Piechota
Plant Breeding and Acclimatization Institute – National Research Institute, Błonie, Poland
Correspondence
Urszula Piechota, Plant Breeding and Acclimatization Institute – National Research Institute, Radzików, 05-870 Błonie, Poland.
Email: u.piechota@ihar.edu.pl
Search for more papers by this authorPiotr Słowacki
Plant Breeding and Acclimatization Institute – National Research Institute, Błonie, Poland
Search for more papers by this authorPaweł C. Czembor
Plant Breeding and Acclimatization Institute – National Research Institute, Błonie, Poland
Search for more papers by this authorAbstract
One of the most important diseases of barley (Hordeum vulgare) is powdery mildew, caused by Blumeria graminis f. sp. hordei. Spring barley line 173-1-2 was selected from a Moroccan landrace and revealed broad-spectrum resistance to powdery mildew. The objective of this study was to map and characterize the gene for seedling powdery mildew resistance in this line. After crossing with the susceptible cultivar ‘Manchuria’, genetic analysis of F2 and F3 families at the seedling stage revealed powdery mildew resistance in line 173-1-2 conditioned by a single recessive gene. Molecular analysis of non-segregating homozygous resistant and homozygous susceptible F2 plants conducted on the DArTseq platform (Diversity Arrays Technology Pty Ltd) identified significant markers which were converted to allele-specific PCR markers and tested among 94 F2 individuals. The new resistance gene was mapped on the long arm of chromosome 6H. No other powdery mildew recessive resistance gene has been located on 6H so far. Therefore, we concluded that the 173-1-2 barley line carries a novel recessive resistance gene designated as mlmr.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
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