Extracellular matrix (ECM)-derived scaffolds show promise in a variety of tissue engineering applications. In article number 2000307, Cathal J. Kearney and co-workers exploit ECM grown by cells differentiated from induced pluripotent stem cells for scaffold fabrication and examine their potential for diabetic wound healing. This ECM has previously been shown to be enriched in foetal-like components, which can augment healing.

Targeting areas of inflammation stands to transform the capacity to effectively treat inflammatory bowel disease while minimizing systemic side effects. In article number 2000536, Giovanni Traverso and co-workers describe heparin-coated human serum albumin nanoparticles that can encapsulate drug combination, suppress inflammation in macrophages in vitro, and selectively bind to inflamed intestine in vivo in a murine model of acute colitis.

In article number 2000538 by Horacio Cabral and co-workers, flexible catiomers fasten mRNA through efficient polyion complexation to form stable mRNA-loaded nanocarriers based on polyion complexation, resulting in enhanced protection against nuclease attack and polyanions toward increased translation both in vitro and in vivo and enhanced bioavailability of mRNA in blood. These results indicate polycation flexibility as a new approach to stabilize polyion complex for enhanced mRNA delivery.

In article number 2000879 by Yafei Feng, Ying Mao, Tiger H. Tao, and co-workers, a new method is reported for preparing injectable silk gel scaffolds inspired by the liquid crystalline spinning process in natural silk fibers, with controllable porosity and sufficient mechanical strength that can be used for minimally invasive intracranial and osteological therapies. Meanwhile, silk gel scaffolds with 3D spatial patterning of porous materials and bioactive agent gradients are achieved.

Commercialization of Avance, a processed nerve allograft, has changed the landscape of the nerve repair market and has treated tens of thousands of patients. This article reviews the events that have brought Avance from the laboratory bench to the patient bedside. Additionally, this review provides a perspective on lessons and considerations that can assist in translation of future medical products.

A schematic representation of the applications of nanomedicine in the diagnosis and treatment of atherosclerosis and related ischemic heart diseases is given. Many applications, such as drug delivery, non-invasive cardiovascular imaging, cardiac tissue engineering, and cardiac biomarker detection have been realized using a variety of nanotechnology-based approaches, including nanoparticles, nanostructured scaffolds, and nanosensors.

Targeting areas of inflammation stands to transform the capacity to effectively treat inflammatory bowel disease while minimizing systemic side effects. This report describes heparin-coated human serum albumin nanoparticles that can encapsulate drug combination, suppress inflammation in macrophages in vitro, and selectively bind to inflamed intestine in vivo in a murine model of acute colitis.

Flexible catiomers fasten mRNA through efficient polyion complexation to form stable mRNA-loaded nanocarriers based on polyion complexation, resulting in enhanced protection against nuclease attack and polyanions toward increased translation both in vitro and in vivo and enhanced bioavailability of mRNA in blood. These results indicate polycation flexibility as a new approach to stabilize PIC for enhanced mRNA delivery.

Effective carbon dots, which have intrinsic nucleolus-targeting capability, high reactive oxygen species generation efficiency and fluorescence quantum yield, excellent biocompatibility, and rapid renal clearance, are proposed and demonstrated for in vitro and in vivo image-guided photodynamic anticancer therapy with enhanced treatment performance at a low dose of carbon dots and light irradiation.

Fully bioresorbable, wireless, battery-free temperature sensors enable measurements of local internal body temperatures over clinically relevant timeframes and then ultimately disappear completely in the body, thereby eliminating the need for extraction surgeries. Potential applications include monitoring of processes associated with wound healing and managing the effects of hyperthermia and hypothermia therapies.

Extracellular matrix (ECM)-derived scaffolds are shown promise in a variety of tissue engineering applications. This work exploits, for the first time, ECM grown by cells differentiated from induced pluripotent stem cells for scaffold fabrication and examines their potential for diabetic wound healing. This ECM has previously been shown to be enriched in foetal-like components, which can augment healing.

A new method is reported for preparing injectable silk gel scaffolds inspired by the liquid crystalline spinning process in natural silk fibers, with controllable porosity and sufficient mechanical strength that can be used for minimally invasive intracranial and osteological therapies. Meanwhile, silk gel scaffolds with 3D spatial patterning of porous materials and bioactive agent gradients can be achieved.

Arrays of field-effect transistors are fabricated from chemical vapor deposition grown graphene and DNA hybridization is detected down to femtomolar concentrations. A thin protection layer prevents contamination of graphene during photolithographic patterning and hybridization. DNA probes are electrostatically immobilized. Hybridization of target DNA induces shifts of the Dirac point. End-point and in situ measurements of hybridization are presented.

Solution-processed conductive polymer composites (CPCs) are patterned on both flexible and stretchable substrates through both drop-coating and direct ink writing (DIW), forming sensitive temperature sensors. The composite ink comprises a high melting point biopolymer matrix (polyhydroxybutyrate [PHB]) and reduced graphene oxide (rGO) nanofiller (from 3 to 12 wt%), and exhibits a negative temperature coefficient of resistance.

A magnetic resonance imaging probe is constructed by co-encapsulation of superparamagnetic Fe₃O₄ nanoparticles and photosensitizer Ce6 in oxidation-responsive micelles for in vivo visual detection of singlet oxygen generated in photodynamic cancer therapy.

Herein, an adhesive colorant is designed by combining coloration with adhesion, which is the property of natural and artificial melanin that allows the localization of enzyme-generated colorants near the target molecule without diffusion to the solution. This localization provides a highly concentrated colorimetric signal with spatial information about the target molecule on the surface.

In theory, the combination of electrostatic precipitation with pressurized intraperitoneal chemotherapy (PIPAC), termed ePIPAC, can result in better tissue penetration of the aerosol. This theoretical advantage is confirmed in this project using a computational fluid dynamics model, an in vitro box model, and an in vivo rat model. Moreover, the peritoneal integrity is not affected by the applied electrostatic forces.

An ambulatory photo-biomodulation therapy using a seamless, human oral motion-powered Smart Dental Implant (SDI) is presented. Under chewing and brushing motions, the piezoelectric dental crown activates a micro-light emitting diodes (LED) via an associate circuitry. Using primary human gingival keratinocytes as a model host and P. aeruginosa LPS as a model inflammatory stimulus, SDI induced efficacious photo-biomodulation (PBM) therapy.

Innovative ultrathin carriers for corneal endothelial cells are developed that consisted of a polyester base and a top layer of gelatin. The membranes supported the characteristic hexagonal morphology and correct phenotype of corneal endothelial cells while exhibiting similar growth rates as the positive control. As a consequence, the proposed membranes prove to be a promising synthetic scaffold for endothelial transplantation.

Current models of in vitro brain development fail to mimic mechanisms in vivo, partly as they use animal-derived matrices. The current study incorporates differentiated cell lines in a fabricated synthetic hydrogel in a microfluidics plate to recapitulate perineural vascular plexus in vivo. By analyzing endpoints such as growth factors, metabolites and 3D cell migration, this model predicts acute toxicity mechanisms.